|
KMID : 0545120040140020395
|
|
Journal of Microbiology and Biotechnology
2004 Volume.14 No. 2 p.395 ~ p.400
|
|
|
An Immobilized Culture of Choristoneura fumiferana Cells for High Production of CfMNPV
|
|
SON, JEONG HWA
BUCHHOLIZ, RAINER/KIM, SUNG-KOO
|
|
|
Abstract
|
|
|
|
Choristnneuru fumiferanu (Cf-2C 1 ) insect cells were cultured and immobilized by using cellulosesulfate (NaCS) and polydiallyldimethylammoniumchloride (PDADMAC). A concentration of´ CNNPV (Chorisroneuru fumiferLznu multiple- nucleopolyhedrovirus) and a Cf-2C I cell density in the microspheres have been achieved at the densities of 1.57x 10^(10) PIBs/ml and 7.5×10^(7)cells/ml, respectively. Additionally, MTT-test was used to measure the viable cell density in the microspheres, and the confidence of MTT-test was investigated before and after baculovirus infection in the immobilized cell culture.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|